Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Analysis of Non-Human Primate Pancreatic Islet…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
Analysis of Non-Human Primate Pancreatic Islet Oxygen Consumption
Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae
Journal (Developmental Biology)
Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae
Investigating mRNA Spatial Distribution in Drosophila Muscle Tissue (Video) | JoVE
Journal (Developmental Biology)
Investigating mRNA Spatial Distribution in Drosophila Muscle Tissue (Video) | JoVE
 

Analysis of Non-Human Primate Pancreatic Islet Oxygen Consumption

Article DOI: 10.3791/60696-v • 07:12 min • December 18th, 2019
December 18th, 2019 •
1, 2, 3, 1,4,5,6
1Department of Molecular Physiology and Biophysics, Vanderbilt University, 2Agilent Technologies, 3Department of Biological Sciences, Vanderbilt University, 4Department of Veterans Affairs Tennessee Valley, 5Department of Medicine, Vanderbilt University Medical Center, 6Department of Cell and Developmental Biology, Vanderbilt University

Chapters

Summary
Automatic Translation

This protocol demonstrates the accurate and reproducible measurement of oxygen consumption in non-human primate pancreatic islets. The islet loading techniques and coating of the microplate provide a framework for efficient measurement of respiration in other types of cultured spheroids.

Tags

Non-human Primate Pancreatic Islet Oxygen Consumption Quantification Mitochondrial Function Small Numbers Of Islets Primary Islets Multiple Conditions Rigor Reproducibility Mitochondrial Health Insulin Secretion Diabetes Islet Transplantation Assay Procedure Valerie Ricciardi Undergraduate Student Cell And Tissue Adhesive Sodium Bicarbonate Solution Spheroid Microplate
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter