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JoVE Journal
Developmental Biology

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Generation of Mosaic Mammary Organoids by Differential Trypsinization
 

Generation of Mosaic Mammary Organoids by Differential Trypsinization

Article doi: 10.3791/60742
March 11th, 2020

Summary March 11th, 2020

The mammary gland is a bilayered structure, comprising outer myoepithelial and inner luminal epithelial cells. Presented is a protocol to prepare organoids using differential trypsinization. This efficient method allows researchers to separately manipulate these two cell types to explore questions concerning their roles in mammary gland form and function.

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