Myoepithelial Cells
Myoepithelial Cells - Related Videos
07:15
Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands
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Cited by 11 •
2019
The lacrimal gland (LG) has two cell types expressing α-smooth muscle actin (αSMA): myoepithelial cells (MECs) and pericytes. MECs are of ectodermal origin, found in many glandular tissues, while pericytes are vascular smooth muscle cells of endodermal origin. This protocol isolates MECs and pericytes from murine LGs.
13:08
Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture
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Cited by 80 •
2013
A method to process human mammary surgical discard material is described. Processed tissue, in the form of organoids, can be stored frozen indefinitely or placed in culture for long-term growth. This method enables experimental examination of normal human epithelial cell biology, and the effects of exogenous perturbations.
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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2025
This protocol describes the in vivo reprogramming of mouse cancer cells into type 1 dendritic-like cells within the tumor microenvironment through enforced expression of the transcription factors PU.1, IRF8, and BATF3.
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 8 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
10:54
Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
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Cited by 2 •
2012
Metaphase to anaphase transition is triggered through anaphase-promoting complex (APC/C)-dependent ubiquitination and subsequent destruction of cyclin B. Here, we established a system which, following pulse-chase labeling, allows monitoring cyclin B proteolysis in entire cell populations and facilitates the detection of interference by the mitotic checkpoint.
FAQs
Their contractile activity depends on an actin and myosin rich cytoskeleton. When these cells receive appropriate stimulation, the cytoskeleton contracts and reduces the space around glandular structures such as acini. This compression helps propel secretions toward ducts, linking cellular contraction with efficient fluid movement through glandular tissue.
Their location between glandular epithelial cells and the basement membrane places them at a strategic interface within the tissue. From this position, contraction can affect the surrounding glandular structure while their organization also contributes to tissue architecture. Examining this arrangement helps researchers relate cellular position to gland development and function.
Myoepithelial cells occur in mammary, salivary, and sweat glands, where their shared contractile behavior supports movement of glandular secretions. The surrounding tissue and gland structure may differ, but studying these locations provides a way to examine how a common cellular property operates across distinct glandular systems in biology and tissue research.
Organization provides information about the relationship between myoepithelial cells, glandular epithelial cells, and neighboring stromal cells. In breast tissue research, assessing that arrangement can reveal changes in tissue architecture associated with disease, including breast cancer. The analysis therefore connects cellular organization with broader alterations in the tissue environment.
Researchers assess these cells through a combination of distinctive morphology and molecular markers rather than relying on location alone. They can also examine interactions with neighboring epithelial and stromal cells. Using these complementary features helps characterize myoepithelial organization and supports comparisons among developing, normal, and disease-associated glandular tissues.
Investigating their morphology, molecular markers, organization, and cellular interactions can clarify several levels of gland biology. These observations may inform understanding of gland development, tissue architecture, and disease-related changes. Because the cells participate in secretion movement as well as structural organization, their assessment connects gland function with the condition of the surrounding tissue.