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Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy
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Developmental Biology
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JoVE Journal Developmental Biology
Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy

Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy

DOI:
10.3791/60744-v

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07:35 min

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February 02, 2020

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, , ,
1Department of Pathology, Department of Oncological Sciences, and Huntsman Cancer Institute,University of Utah School of Medicine

Chapters

  • 00:05Introduction
  • 00:49Fixing Larvae and Dissecting Tissues Surrounding Liver
  • 02:53Imaging
  • 04:05Image Analysis
  • 06:07Results: Zebrafish Larvae
  • 06:53Conclusion

Summary

Automatic Translation

Automatic Translation

Here we demonstrate a method for quantifying liver size in larval zebrafish, providing a way to assess the effects of genetic and pharmacologic manipulations on liver growth and development.

Tags

Keywords: Liver SizeLarval ZebrafishBrightfield MicroscopyOrgan QuantificationGenetic ManipulationPharmacologic ManipulationLiver GrowthLiver DevelopmentDissectionIn Situ HybridizationConfocal ImagingEuthanasiaPBSPFASkin RemovalYolk RemovalForcepsGlass Dish

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