Zebrafish Larvae
Zebrafish Larvae - Related Videos
10:53
Microgavage of Zebrafish Larvae
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Cited by 91 •
2013
We present a novel method for microgavage of larval zebrafish utilizing standard embryo microinjection and stereomicroscopy equipment. We demonstrate that microgavage is a safe and efficient technique useful for delivering controlled amounts of diverse materials specifically into the larval zebrafish intestinal lumen.
02:28
Inducing Neuroinflammation in Zebrafish Larvae with a Lipopolysaccharide Injection
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2025
The video demonstrates the microinjection of lipopolysaccharides (LPS) into the brains of zebrafish larvae to induce neuroinflammation. The larvae are positioned on an agarose-lined dish, the needle is inserted into the brain ventricles, and the LPS is injected. The injected LPS triggers an immune response that leads to neuroinflammation.
02:24
A Technique to Quantify Fungal Infection in Zebrafish Larvae
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2025
This video demonstrates a method to quantify fungal infection in zebrafish larvae. Fungal spores are microinjected into the larval hindbrain ventricle, and the larvae are incubated to allow infection development. Infected larvae are harvested at defined intervals, and infection progress is assessed by enumerating colony-forming units.
06:14
Automating Tumor Implantation in Zebrafish Larvae for Cancer Research and Medicine
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2025
Here, we present a detailed protocol for automated tumor xenograft implantation into zebrafish larvae using a state-of-the-art robotic injection system. Furthermore, we present injection videos and validation data demonstrating the system's successful performance.
02:56
Modeling Intestinal Infection in Zebrafish Larvae Using Foodborne Bacteria
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2026
Source: Flores, E., et al. Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae. J. Vis. Exp. (2019).This video demonstrates the method of establishing a foodborne bacterial intestinal infection model in zebrafish larvae by feeding them paramecia infected with fluorescent E. coli.
FAQs
External development makes developmental events accessible without requiring researchers to infer them solely from internal anatomy. Because the embryos and larvae remain largely transparent, investigators can monitor cell division, organ formation, tissue differentiation, and emerging circulation in living animals. This combination connects visible structural changes with the timing of conserved vertebrate developmental processes.
The yolk provides support while rapid cell division and organ formation proceed after fertilization. Coordinated signaling then contributes to tissue differentiation, and emerging circulation helps shape the larval body and behavior. Studying these components together emphasizes that development depends on interacting processes, not on cell division alone.
Their conservation gives observations in zebrafish larvae broader biological relevance: developmental changes can be examined in a vertebrate context rather than treated as species-specific events. This is particularly useful when investigators assess gene function or study disease mechanisms, because the larval system links experimentally accessible development with processes shared across vertebrates.
Behavioral changes add a functional readout to structural development. As signaling, tissue differentiation, and circulation contribute to the forming larval body, behavior can emerge alongside anatomical change. Monitoring both dimensions helps biology studies relate developmental mechanisms to observable outcomes, rather than evaluating organ formation or tissue patterning without considering the animal's developing function.
A basic observational workflow can follow living larvae through developmental change, using their optical accessibility to monitor visible events. Researchers can then connect those observations with assessments of gene function or with measured responses to drugs, toxins, and environmental changes. The compact format supports biological observation across these research purposes.
Drug, toxin, and environmental-change studies can treat larval responses as measurable biological outcomes. Investigators measure how living larvae respond to these factors, using their small size and optical accessibility to observe effects in an intact developing animal. The resulting observations support toxicology and therapeutic discovery while preserving developmental context.
Zebrafish larvae are useful when a study must connect developmental biology with broader biological questions. Their accessible development supports work on disease mechanisms and neurobiology, while response measurements extend their use to toxicology and therapeutic discovery. This range allows one model to link formation of the body with disease-mechanism questions, nervous-system questions, and intervention-focused research.