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JoVE Journal Neuroscience
Long-range Channelrhodopsin-assisted Circuit Mapping of Inferior Colliculus Neurons with Blue and Red-shifted Channelrhodopsins
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Journal (Neuroscience)
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Long-range Channelrhodopsin-assisted Circuit Mapping of Inferior Colliculus Neurons with Blue and Red-shifted Channelrhodopsins

Article DOI: 10.3791/60760-v • 07:04 min • February 7th, 2020
February 7th, 2020 •
1, 1
1Kresge Hearing Research Institute, Department of Otolaryngology - Head and Neck Surgery, University of Michigan

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Summary
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Channelrhodopsin-assisted circuit mapping (CRACM) is a precision technique for functional mapping of long-range neuronal projections between anatomically and/or genetically identified groups of neurons. Here, we describe how to utilize CRACM to map auditory brainstem connections, including the use of a red-shifted opsin, ChrimsonR.

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Long-range Channelrhodopsin-assisted Circuit Mapping Inferior Colliculus Neurons Blue-shifted Channelrhodopsins Red-shifted Channelrhodopsins Functional Mapping Auditory Brain Stem CRACM Lambda Stereotaxic Coordinate System Surgery Area Sanitation Sterile Towel Drapes Cage Bedding Removal Heating Pad Food And Water Source Animal Transfer Anesthesia Continuation Rectal Temperature Probe Homeostatic Temperature Controller Ophthalmic Ointment Application Preemptive Analgesic Administration Scalp Shaving Incision In The Scalp
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