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High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
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High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

Article DOI: 10.3791/60800-v • 08:18 min • June 16th, 2020
June 16th, 2020 •
1,2, 1, 3, 1,2, 1,2
1Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, 2Graduate School of Frontier Biosciences, Osaka University, 3Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford

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Summary
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Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software, Chromagnon.

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High-Accuracy Correction 3D Chromatic Shifts Super-Resolution Biological Imaging ChromagnonMarsh Coral Microscopy Images Colocalization Analysis High Resolution Images Correct Chromatic Shift Biological Sample Reference Images Takako Koujin Healer Cells Carbon Dioxide Incubator Formaldehyde PBS Permeabilize Cells Trypsin In PBS Antibody Staining Protocol Crosstalk Reference Widefield Microscopy DAPI Staining
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