Super Resolution Imaging
Super Resolution Imaging - Related Videos
08:47
Super-resolution Imaging of the Bacterial Division Machinery
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Cited by 17 •
2013
We describe a super-resolution imaging method to probe the structural organization of the bacterial FtsZ-ring, an essential apparatus for cell division. This method is based on quantitative analyses of photoactivated localization microscopy (PALM) images and can be applied to other bacterial cytoskeletal proteins.
09:30
Super-resolution Imaging of Neuronal Dense-core Vesicles
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Cited by 3 •
2014
We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution image.
07:55
Ground State Depletion Super-resolution Imaging in Mammalian Cells
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Cited by 16 •
2017
This article outlines a protocol for the detection of one or more plasma and/or intracellular membrane proteins using Ground State Depletion (GSD) super-resolution microscopy in mammalian cells. Here, we discuss the benefits and considerations of using such approaches for the visualization and quantification of cellular proteins.
10:01
Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
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Cited by 8 •
2017
The use of a hyperlens has been regarded as a novel super-resolution imaging technique due to its advantages in real-time imaging and its simple implementation with conventional optics. Here, we present a protocol describing the fabrication and imaging applications of a spherical hyperlens.
08:43
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
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Cited by 5 •
2017
We demonstrate a method to image multiple molecules within heterogeneous nano-structures at single molecule accuracy using sequential binding and elution of fluorescently labeled antibodies.
FAQs
The approach manages fluorescent signals so that neighboring emitters can be separated rather than appearing as one blurred feature. Depending on the technique, this separation may rely on single-molecule localization, optical signal control, or structured illumination. Reducing the effective blur allows researchers to distinguish organization that conventional light microscopy leaves unresolved.
These approaches represent different ways to obtain finer spatial information from fluorescent samples. Single-molecule localization separates signals from individual emitters, while stimulated emission depletion and structured illumination use optical control of fluorescence or illumination patterns. Their shared purpose is to distinguish nearby signals and reveal nanoscale biological organization.
Fluorescent-signal control determines whether closely positioned molecules remain distinguishable in the recorded image. By regulating or separating signals from neighboring emitters, the method can resolve distributions and interactions that would otherwise merge into a single feature. This capability is central to connecting molecular-scale organization with the structure and function of cells.
Applications include protein distributions, organelles, membranes, and cytoskeletal organization. Imaging these targets at finer spatial scales can show how components are arranged within cells and how that arrangement relates to cellular function. The same general capability also supports investigation of molecular interactions and dynamic behavior rather than only broad cellular morphology.
Fixed specimens support examination of cellular organization at a selected state, whereas living specimens allow researchers to follow molecular dynamics over time. This distinction lets investigators choose between resolving a preserved structural arrangement and observing changing behavior. Both contexts can contribute complementary information about how nanoscale organization relates to cell function.
Biologists apply it when the relationship between nanoscale structure and biological activity is important. The technique supports research in cell biology, neuroscience, development, and disease mechanisms by mapping protein distributions, examining organelles or membranes, and tracking molecular dynamics. These uses help investigate how altered organization may relate to changes in cellular behavior.