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JoVE Journal
Genetics

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Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
 

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Article DOI: 10.3791/60808-v 06:46 min
April 2nd, 2020

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Summary April 2nd, 2020

Here, we present a modified method for cryopreservation of one-cell embryos as well as a protocol that couples the use of freeze-thawed embryos and electroporation for the efficient generation of genetically modified mice.

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