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Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
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Bioengineering
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JoVE Journal Bioengineering
Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion

DOI:
10.3791/60816-v

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08:34 min

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February 24, 2021

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, ,
1Department of Plastic, Reconstructive and Aesthetic Surgery, Geneva University Hospitals, Faculty of Medicine,Geneva University, 2Regen Lab SA

Chapters

  • 00:04Introduction
  • 01:01Preparation of Platelet‐Rich Plasma (PRP)
  • 02:02Isolation and Culture of Autologous Fibroblasts
  • 06:13Results: Standardized 1.5X Leukocyte Poor PRP Boosts Cell Proliferation
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents a device that produces PRP to boost the in vitro expansion of cells in a 100% autologous fibroblast culture system.

Tags

Keywords: Autologous Platelet-rich PlasmaFibroblast ExpansionCell CultureCell TherapyCollagenase/dispaseCentrifugationPRP Preparation

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