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JoVE Journal
Chemistry
Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single ...
Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single ...
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Full Text
18,672 Views
07:24 min
April 14, 2020

DOI: 10.3791/60826-v

Emille M. Rodrigues1, Nelson Rutajoga1, David Rioux2, Jacob Yvon-Leroux2, Eva Hemmer1

1Department of Chemistry and Biomolecular Sciences,University of Ottawa, 2Photon etc

Here, we present a protocol to obtain luminescent hyperspectral imaging data and to analyze optical anisotropy features of lanthanide-based single crystals using a Hyperspectral Imaging System.

Hyperspectral imaging allows for the acquisition of spectroscopic information at precise locations within a sample. For example, for the unveiling of optical anisotropy at a single crystal level. The generation of both spectral and spatial data of a sample allows for a more detailed investigation of the sample than is possible by either spectroscopy or fluorescence microscopy alone.

A large number of adjustable parameters which affect the resolution can seem overwhelming at first. However, making a checklist may help with familiarizing yourself with the system. This technique requires manual tuning of the imaging hardware as well as software manipulation.

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