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DOI: 10.3791/60826-v
Here, we present a protocol to obtain luminescent hyperspectral imaging data and to analyze optical anisotropy features of lanthanide-based single crystals using a Hyperspectral Imaging System.
Hyperspectral imaging allows for the acquisition of spectroscopic information at precise locations within a sample. For example, for the unveiling of optical anisotropy at a single crystal level. The generation of both spectral and spatial data of a sample allows for a more detailed investigation of the sample than is possible by either spectroscopy or fluorescence microscopy alone.
A large number of adjustable parameters which affect the resolution can seem overwhelming at first. However, making a checklist may help with familiarizing yourself with the system. This technique requires manual tuning of the imaging hardware as well as software manipulation.
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