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Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)
JoVE Journal
Genetics
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JoVE Journal Genetics
Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)
DOI:

08:12 min

March 11, 2020

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Chapters

  • 00:04Introduction
  • 01:01Tartary Buckwheat (TB) Seed Preparation
  • 01:49TB Seedling and Explant Preparation
  • 02:46A. rhizogenes Transformation Preparation
  • 03:56TB Explant Screening, Infection, and Co-Culture
  • 04:47Induction and Selective Culture and TB Hairy Root Subculture
  • 05:32Transformed Hairy Root Identification and Conservation
  • 06:14Results: Representative Hairy Root Transformation Identification and Characterization
  • 07:23Conclusion

Summary

Automatic Translation

We describe a method of inducing hairy roots by Agrobacterium rhizogenes-mediated transformation in Tartary buckwheat (Fagopyrum tataricum). This can be used to investigate gene functions and production of secondary metabolites in Tartary buckwheat, be adopted for any genetic transformation, or used for other medicinal plants after improvement.

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