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Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer
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1Laboratory for Targeted Drug Delivery and Personalized Medicine Technologies, Department of Chemical Engineering,Technion – Israel Institute of Technology, 2The Norman Seiden Multidisciplinary Program for Nanoscience and Nanotechnology,Technion – Israel Institute of Technology, 3The Interdisciplinary Program for Biotechnology,Technion – Israel Institute of Technology
Chapters
- 00:04Introduction
- 00:48Preparation of S30-T7 Lysate
- 04:24Preparation of Lipids-in-Oil Solution
- 05:08Preparation of Synthetic Cells
- 07:24Results: Production of sfGFP and Renilla Luciferase
- 08:57Conclusion
This protocol describes the method, materials, equipment and steps for bottom-up preparation of RNA and protein producing synthetic cells. The inner aqueous compartment of the synthetic cells contained the S30 bacterial lysate encapsulated within a lipid bilayer (i.e., stable liposomes), using a water-in-oil emulsion transfer method.
