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Journal / Biochemistry / In Vivo Hydroxyl Radical Protein Footprinting zur …
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Biochemistry

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JoVE Journal Biochemistry
In Vivo Hydroxyl Radical Protein Footprinting zur Untersuchung von Protein-Wechselwirkungen bei Caenorhabditis elegans
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In Vivo Hydroxyl Radical Protein Footprinting zur Untersuchung von Protein-Wechselwirkungen bei Caenorhabditis elegans

Article DOI: 10.3791/60910-v • 08:00 min • April 1st, 2020
April 1st, 2020 •
1, 1
1Department of Pharmaceutical Sciences, University of Maryland

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In vivo schnelle photochemische Oxidation von Proteinen (IV-FPOP) ist eine Hydroxyl-Radikal-Protein-Footprinting-Technik, die die Kartierung der Proteinstruktur in ihrer heimischen Umgebung ermöglicht. Dieses Protokoll beschreibt die Montage und Einrichtung des mikrofluidischen Strömungssystems IV-FPOP.

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Biochemie Ausgabe 158 Caenorhabditis elegans Hydroxyl-Radikal-Protein-Fußabdruck FPOP Massenspektrometrie in vivo Proteomik
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