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Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments
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JoVE Journal Neuroscience
Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments

Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments

DOI:
10.3791/60912-v

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09:05 min

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February 09, 2020

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, , , , ,
1Sorbonne Université, Inserm, CNRS, UMR7225,Institut du Cerveau et de la Moelle épinière, ICM, 2Assistance Publique-Hôpitaux de Paris,GH Pitié Salpêtrière, 3Ecole Supérieure des Techniques de Biologie Appliquée

Chapters

  • 00:04Introduction
  • 01:24Dissection
  • 02:23Tissue Dissociation
  • 04:13OL (Oligodendrocyte) Lineage Cells Harvesting and Culture
  • 06:30OCM (Oligodendrocyte-conditioned Medium) Production
  • 07:10Results: OL Lineage Cells Phenotype and Representative Applications
  • 08:20Conclusion

Summary

Automatic Translation

Automatic Translation

Herein, we display an efficient method for the purification of oligodendrocytes and production of oligodendrocyte-conditioned medium that can be used for co-culture experiments.

Tags

Keywords: OligodendrocytesOligodendrocyte-conditioned MediumCo-cultureMultiple SclerosisMyelinationCell CultureCerebral CorticesEnzymatic DigestionCell Filtration

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