A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Institute of Molecular Cardiology, Department of Medicine,University of Louisville, 2Department of Bioengineering,University of Louisville, 3Diabetes and Obesity Center, Department of Medicine,University of Louisville, 4Department of Pharmacology and Toxicology,University of Louisville, 5Institute of Cardiovascular Sciences,University of Manchester, 6Faculty of Pharmacy,Zagazig University
Chapters
- 00:05Introduction
- 00:49Pig Heart Tissue Slicing
- 02:35Slice Position Adjustment
- 03:27Slice Collection
- 04:03Heart Slice Culture
- 06:19Results: Pig Heart Slice Culture Viability and Functionality Validation
- 07:06Conclusion
Denne protokollen beskriver hvordan å skjære og kultur hjertevev under fysiologiske forhold i 6 dager. Dette kultursystemet kan brukes som en plattform for å teste effekten av nye hjertesvikt terapeutiske samt pålitelig testing av akutt kardiotoksisitet i en 3D hjertemodell.
