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Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro

Article DOI: 10.3791/60952-v • 08:16 min • May 20th, 2020
May 20th, 2020 •
1, 1, 1, 1, 1
1Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Nantong University

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Summary
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Here, we present a method to purify fibroblasts and Schwann cells from sensory and motor nerves in vitro.

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Purification Fibroblasts Schwann Cells Sensory Nerves Motor Nerves In Vitro Large Number Nerve Regeneration Nervous System Diseases Experimental Steps Differential Digestive Step Scissors Incision Skin Spinal Column Vertebral Canal Spinal Cord Petri Dish D Hanks Balanced Salt Solution Ventral Root Dorsal Root Nerve Pieces Trypsin Centrifuge Tubes Incubate DMEM Fetal Bovine Serum
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