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In Situ Påvisning af Ribonucleoprotein Complex Assembly i C. elegans Germline ved hjælp af Nærhed Ligation Assay
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
DOI:

08:56 min

May 05, 2020

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Chapters

  • 00:04Introduction
  • 00:49Dissection/Gonad Extrusion
  • 02:46Fixation/Blocking
  • 04:14Primary Antibody Incubation and PLA Probe (Secondary Antibody) Incubation
  • 05:21Ligation
  • 06:09Amplification and Final Washes
  • 06:45Coverslip Mounting
  • 07:15Results: Germlines Following Co-immunostaining or PLA
  • 08:10Conclusion

Summary

Automatic Translation

Denne protokol viser brugen af nærhedligation assay at sonde for protein-protein interaktioner in situ i C. elegans germline.

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