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Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture
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Neuroscience
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JoVE Journal Neuroscience
Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

DOI:
10.3791/60989-v

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09:40 min

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April 02, 2020

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, , ,
1Program in Behavioral Neuroscience, Department of Psychology,University of South Carolina

Chapters

  • 00:04Introduction
  • 00:38Preparation of Dil/Tungsten Bead Tubing
  • 02:55Preparation of Brain Sections
  • 04:20Ballistic Labeling and Visualization of Brain Sections
  • 06:16Cell Culture Method
  • 07:12Results: Analysis of Pyramidal Neurons Using Ballistic Labeling Technology
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to label and analyze pyramidal neurons, which is critical for evaluating potential morphological alterations in neurons and dendritic spines that may underlie neurochemical and behavioral abnormalities.

Tags

Ballistic LabelingPyramidal NeuronsBrain SlicesPrimary Cell CulturePVP SolutionTungsten Microcarrier BeadsLipophilic DiIC18(3) DyeMethylene ChlorideSonicationNitrogen Gas DryingRat Brain PerfusionPBSPFA

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