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Cancer Research
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胶质母细胞瘤的3D球形模型
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
A 3D Spheroid Model for Glioblastoma
Please note that all translations are automatically generated.
Click here for the English version.
胶质母细胞瘤的3D球形模型
DOI:
10.3791/60998-v
•
07:40 min
•
April 09, 2020
•
Joris Guyon
2
,
Laetitia Andrique
2
,
Nadège Pujol
2
,
Gro Vatne Røsland
4
,
Gaelle Recher
5,6
,
Andreas Bikfalvi
2
,
Thomas Daubon
2,4
1
University Bordeaux, LAMC
,
2
INSERM U1029, University Bordeaux
,
3
Department of Biomedicine
,
University of Bergen
,
4
CNRS, IBGC UMR5095
,
5
LP2N, CNRS UMR 5298, IOA
,
6
Institut d’Optique Graduate School, IOA
Chapters
00:05
Introduction
01:04
Uniformly Sized Tumor Spheroid Generation
02:12
Invasion
03:07
Migration
04:03
Image Acquisition and Analysis
04:57
Results: Representative Spheroid Analyses
06:49
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一种容易使用的胶质细胞瘤入侵测定。这种测定适用于胶质母细胞瘤干细胞。还介绍了斐济宏,用于轻松量化入侵、移徙和扩散。
Tags
3D Spheroid Model
Glioblastoma
3D Cell Culture
Tumor Invasion
Tumor Migration
Collagen Matrix
Matrigel
Tumor Heterogeneity
Drug Evaluation
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