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Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
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Biochemistry
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JoVE Journal Biochemistry
Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

DOI:
10.3791/61005-v

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06:07 min

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June 07, 2020

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, ,
1Department of Anthropology,Indiana University

Chapters

  • 00:04Introduction
  • 00:50Extraction of Potential Plant Phytosteroids
  • 02:29Human Estrogen Receptor β Transfection Assay
  • 04:25Results: ERβ Reporter Assay System for Phytoestrogen Activity Screening of Fruits and Vegetables
  • 05:19Conclusion

Summary

Automatic Translation

Automatic Translation

We have optimized a commercially available estrogen receptor β reporter assay for screening human and nonhuman primate foods for estrogenic activity. We validated this assay by showing that the known estrogenic human food soy registers high, while other foods show no activity.

Tags

Keywords: PhytoestrogensCell-based AssayEstrogen ReceptorReporter AssayPlant ExtractsLyophilizationMethanol ExtractionDMSOSample PreparationContaminationAseptic Technique

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