Luminometer Detection
Luminometer Detection - Related Videos
11:31
'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
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Cited by 18 •
2011
A simple method for the identification of priority bacterial pathogens is to use genetically engineered reporter phage. These reporter phage, which are specific to their particular host species, are capable of rapidly transducing a bioluminescent signal response to host cells. Herein, we describe the use of reporter phage for the detection of Yersinia pestis.
03:56
An In Vitro Recombinant Virus Reporter System for the Detection of Viral Infection
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2025
This video demonstrates an in vitro technique to detect hepatitis B virus (HBV) infection via a recombinant HBV reporter system. Upon infecting host cells, the recombinant viral genome expresses the luciferase reporter enzyme, which produces bioluminescence upon oxidizing its substrate — helping detect viral infection.
07:17
Production and Detection of Reactive Oxygen Species (ROS) in Cancers
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Cited by 278 •
2011
Here we propose simple methods to test and evaluate the presence of reactive oxygen species in cells.
03:11
Bioreporter Assay: A Sensitive Technique using a Bioluminescent Reporter System to Detect SARS-CoV-2 Antibodies
0 Views •
2025
In this video, we show the HiBit-RBD bioreporter system-based assay for detecting SARS-CoV-2 neutralizing antibodies. This system targets the viral RBD domain by using a bioluminescent reporter system containing HiBiT fused to the RBD domain.
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
FAQs
Inside a light-tight chamber, the instrument collects photons emitted by the reaction and directs them to a photomultiplier tube or similar detector. The detector converts the light into an electrical signal that can be measured. This conversion allows researchers to analyze extremely low light levels and relate the recorded signal to biological or chemical activity.
In a luciferase assay, luciferase catalyzes oxidation of a substrate, producing emitted light. The measured intensity is proportional to the activity represented by the reaction, so the signal can serve as a quantitative readout. This mechanism supports analysis of enzyme activity, cellular responses, and reporter-protein expression in biological samples.
Proportionality connects the amount of detected light with the activity being studied. When a luciferase reaction produces light in proportion to the relevant target activity, differences in electrical signal can be used to quantify differences between samples. This makes the measurement useful not only for detecting a response, but also for comparing biological activity.
A typical measurement places a chemical or biological reaction in the instrument’s light-tight chamber, where emitted photons are collected by the detector. The instrument converts those photons into an electrical signal and records the resulting measurement. In luciferase assays, the reaction includes the enzyme and its substrate, allowing the recorded signal to represent the activity under study.
The method can quantify ATP, monitor gene expression through reporter proteins, measure enzyme activity, and detect cellular responses. These readouts connect emitted light with distinct biological processes rather than limiting the technique to a single assay type. Its high sensitivity is particularly valuable when the relevant reaction produces only very low levels of light.
Luminometer Detection supports drug screening, diagnostics, and environmental analysis in addition to molecular biology research. In these settings, sensitive measurement of reaction-generated light can help quantify a biological signal, assess enzyme or cellular activity, or detect a response in a sample. The same photon-to-electrical measurement principle therefore serves multiple analytical contexts.