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Journal
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Bioengineering
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原子力显微镜在早期骨关节炎检测中的应用
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Application of Atomic Force Microscopy to Detect Early Osteoarthritis
Please note that all translations are automatically generated.
Click here for the English version.
原子力显微镜在早期骨关节炎检测中的应用
DOI:
10.3791/61041-v
•
09:22 min
•
May 24, 2020
•
Marina Danalache*
1
,
Aadhya Tiwari*
1
,
Viktor Sigwart
2
,
Ulf Krister Hofmann
3
1
Laboratory of Cell Biology, Department of Orthopaedic Surgery
,
University Hospital of Tübingen
,
2
Medical faculty of the University of Tübingen
,
3
Department of Orthopaedic Surgery
,
University Hospital of Tübingen
Chapters
00:04
Introduction
00:47
Cartilage Cryotome Sectioning
01:36
Cartilage Sample Atomic Force Microscopy (AFM) Preparation
02:17
AFM Device Preparation
03:14
Cantilever Sample and Calibration
05:25
Biomechanical Extracellular Matrix (ECM) and Pericellular Matrix (PCM) Characterization
07:04
Data Processing
07:46
Results: Representative Relationships Between Young s Moduli and Cellular Spatial Organization
08:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一种使用原子力显微镜(AFM)研究关节软骨细胞水平上早期骨关节炎变化的方法。
Tags
Atomic Force Microscopy
Osteoarthritis
Biomechanical Properties
Elasticity
Articular Cartilage
Cryotome
PBS
Sample Glue
Leibovitz’s L-15 Medium
AFM Cantilever
Microsphere Probe
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