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Immunology and Infection

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JoVE Journal Immunology and Infection
Flöde Cytometric Analys av lymfocyt infiltration i centrala nervsystemet under experimentell autoimmun encefalomyelit
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Flöde Cytometric Analys av lymfocyt infiltration i centrala nervsystemet under experimentell autoimmun encefalomyelit

Article DOI: 10.3791/61050-v • 09:01 min • November 17th, 2020
November 17th, 2020 •
1,2, 1, 3, 1, 3
1Translational Medicine Research Center, Ruijin Hospital North, Shanghai Jiao Tong University School of Medicine, 2Department of Microbiology and Immunology, Tongji University School of Medicine, 3Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine

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Detta manuskript presenterar ett protokoll för att inducera aktiva experimentella autoimmuna encefalomyelit (EAE) i möss. En metod för isolering och karakterisering av de infiltrerade lymfocyterna i centrala nervsystemet (CNS) presenteras också för att visa hur lymfocyter är involverade i utvecklingen av CNS autoimmun sjukdom.

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Immunologi och infektion multipel skleros MS experimentell autoimmun encefalomyelit EAE autoimmun sjukdom centrala nervsystemet CNS T-lymfocyt flödescytometri
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