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Biochemistry

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JoVE Journal Biochemistry
En platform for hydrogendeuteriumudvekslingsmassespektrometri (HDX-MS) til undersøgelse af peptidbiosyntetiske enzymer
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En platform for hydrogendeuteriumudvekslingsmassespektrometri (HDX-MS) til undersøgelse af peptidbiosyntetiske enzymer

Article DOI: 10.3791/61053-v • 11:32 min • May 4th, 2020
May 4th, 2020 •
1, 1
1Department of Chemistry, McGill University

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Lanthipeptide synthetases katalyserer multistep reaktioner under biosyntesen af peptid naturlige produkter. Her beskriver vi en kontinuerlig, bottom-up, hydrogen-deuterium udveksling massespektrometri (HDX-MS) workflow, der kan anvendes til at studere kropsbygning dynamik lanthipeptid synthetaser, samt andre lignende enzymer involveret i peptid naturlige produkt biosyntese.

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Biokemi Hydrogen-deuterium udveksling massespektrometri biokemi enzymologi biosyntese naturlige produkter peptider
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