Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / En Hydrogen-Deuterium Exchange Mass Spectrometry…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biochemistry
En Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) plattform for undersøkelse av peptidbiosyntetiske enzymer
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
Journal (Biochemistry)
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
Journal (Biochemistry)
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
 
Click here for the English version

En Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) plattform for undersøkelse av peptidbiosyntetiske enzymer

Article DOI: 10.3791/61053-v • 11:32 min • May 4th, 2020
May 4th, 2020 •
1, 1
1Department of Chemistry, McGill University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Lanthipeptide synthetases katalyserer multistep reaksjoner under biosyntesen av peptid naturlige produkter. Her beskriver vi en kontinuerlig, nedenfra-og-opp, hydrogen-deuterium utveksling masse spektrometri (HDX-MS) arbeidsflyt som kan brukes til å studere konformasjonsdynamikken til lanthipeptid synthetaser, samt andre lignende enzymer involvert i peptid naturlig produkt biosyntese.

Tags

Biokjemi Utgave 159 Hydrogen-deuterium utveksling massespektrometri biokjemi enzymologi biosyntese naturlige produkter peptider
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter