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Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes
Immunostaining of Whole-Mount <em>Drosophila</em> Testes for 3D Confocal Analysis of Large Spermatocytes
JoVE Journal
Biology
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JoVE Journal Biology
Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes

Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes

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08:37 min

August 27, 2020

DOI:

08:37 min
August 27, 2020

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Summary

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We present here an improved protocol for the whole-mount preparation and immunostaining of Drosophila testes, suitable for confocal microscopy and also allowing reproducible and reliable labeling. We illustrate this protocol by 3D representation and quantification of colocalization experiments on the large S4-S5 spermatocytes.

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