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Silenciamiento de microARN a base de X del virus de la papa (VbMS) en la papa.
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Silenciamiento de microARN a base de X del virus de la papa (VbMS) en la papa.

Article DOI: 10.3791/61067-v • 11:51 min • May 11th, 2020
May 11th, 2020 •
1, 1, 1,2
1Texas A&M AgriLife Research Center at Dallas, Texas A&M University System, 2Department of Plant Pathology, Microbiology, Texas A&M University

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Presentamos un protocolo detallado para el sistema de silenciamiento de microARN (VbMS) basado en el virus de la papa X (PVX) para caracterizar funcionalmente los microARN endógenos (miRNAs) en la papa. Las moléculas de imitación objetivo (TM) de miRNA de interés se integran en el vector PVX y se expresan transitoriamente en la papa para silenciar la familia objetivo de miRNA o miRNA.

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Ciencias Ambientales Número 159 silenciamiento de microARN basado en virus (VbMS) virus de la papa X (PVX) microARN (miRNA) imitación de diana (TM) imitación de diana en tándem corto (STTM) Solanum tuberosum
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