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Potatisvirus X-baserad mikroRNA-ljuddämpning (vbMS) i potatis.
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Potatisvirus X-baserad mikroRNA-ljuddämpning (vbMS) i potatis.

Article DOI: 10.3791/61067-v • 11:51 min • May 11th, 2020
May 11th, 2020 •
1, 1, 1,2
1Texas A&M AgriLife Research Center at Dallas, Texas A&M University System, 2Department of Plant Pathology, Microbiology, Texas A&M University

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Vi presenterar ett detaljerat protokoll för potatisvirus X (PVX)-baserade microRNA ljuddämpning (VbMS) system för att funktionellt karakterisera endogena microRNAs (miRNAs) i potatis. Målimiter (TM) molekyler av miRNA av intresse integreras i PVX vektorn och uttrycks tillfälligt i potatis för att tysta målet miRNA eller miRNA familjen.

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Miljövetenskap utgåva 159 virusbaserad mikroRNA-ljuddämpning (VbMS) potatisvirus X (PVX) mikroRNA (miRNA) målimit (TM) korta tandemmålimiter (STTMs) Solanum tuberosum
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