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为电子生理记录准备急性人类河马切片
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings
Please note that all translations are automatically generated.
Click here for the English version.
为电子生理记录准备急性人类河马切片
DOI:
10.3791/61085-v
•
07:31 min
•
May 07, 2020
•
Larissa Kraus
,
Laura Monni
2
,
Ulf C. Schneider
,
Julia Onken
,
Philipp Spindler
,
Martin Holtkamp
,
Pawel Fidzinski
2
1
Department of Neurology with Experimental Neurology
,
Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health
,
2
NeuroCure Cluster of Excellence
,
Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health
,
3
Department of Neurosurgery
,
Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health
Chapters
00:04
Introduction
00:33
Solution Preparation
02:01
Interface Chamber Preparation
02:39
Preparation Area Setup
03:39
Tissue Slicing and Storing
04:37
Epileptiform Activity Recording
05:59
Results: Representative Epileptiform and Epileptic Burst Activity
06:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出的方案描述了被切除的人类海马组织的运输和制备,最终目标是使用重要的脑片作为潜在抗癫痫物质的临床前评估工具。
Tags
Human Hippocampus
Electrophysiological Recordings
Acute Brain Slices
Anti-epileptic Drug Development
Temporal Lobe Epilepsy
Choline ACSF
Carbogenation
Interface Chamber
Vibratome
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