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Journal / Bioengineering / Skalerbar generation af modne cerebellar…
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JoVE Journal Bioengineering
Skalerbar generation af modne cerebellar organoider fra humane pluripotente stamceller og karakterisering ved immunstaining
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Skalerbar generation af modne cerebellar organoider fra humane pluripotente stamceller og karakterisering ved immunstaining

Article DOI: 10.3791/61143-v • 10:40 min • June 13th, 2020
June 13th, 2020 •
1,2, 1, 1, 1, 1,2,3, 4, 4, 4, 2, 1
1iBB - Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 3The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Universidade de Lisboa, 4PBS Biotech, Inc, Camarillo, CA, USA

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Denne protokol beskriver et dynamisk kultursystem til fremstilling af kontrollerede størrelsesggreg aggregater af humane pluripotente stamceller og yderligere stimulerer differentiering i cerebellar organoider under kemisk definerede og feeder-fri betingelser ved hjælp af en engangsbioreaktor.

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Bioengineering human induceret pluripotente stamceller cerebellar differentiering dynamisk system definerede kulturbetingelser kryosektion immunstaining
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