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同时筛查1型糖尿病和多种自身免疫性疾病的高通量电化学发光7重检测
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
Please note that all translations are automatically generated.
Click here for the English version.
同时筛查1型糖尿病和多种自身免疫性疾病的高通量电化学发光7重检测
DOI:
10.3791/61160-v
•
06:50 min
•
May 29, 2020
•
Xiaofan Jia*
1,2
,
Ling He*
1,3
,
Yong Gu
,
Hilary High
,
Liping Yu
1
Barbara Davis Center for Diabetes
,
University of Colorado School of Medicine
,
2
Department of Endocrinology, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine
,
Chinese Academy of Medical Sciences
,
3
Department of endocrinology, Guangzhou First People’s Hospital, School of Medicine
,
South China University of Technology
Chapters
00:04
Introduction
00:50
Mixed Linker-Coupled Antigen Solution
02:26
Serum Sample Incubation with Labeled Antigen
03:30
Multiplex Plate Preparation
03:57
Serum/Antigen Incubate Transfer
04:58
Results: Representative Autoantibody Level Evaluation
06:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们模拟了一个简单的多重ECL检测,将7种自身抗体检测组合在一起。该测定能够同时筛查 T1D 和多种其他自身免疫性疾病,包括乳糜泻、自身免疫性甲状腺疾病和自身免疫性多腺综合征 1。
Tags
Electrochemiluminescence Assay
Type 1 Diabetes
Autoimmune Diseases
High-throughput
Multiplex
Antigen Labeling
Serum Incubation
Autoantibody Detection
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