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Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices
 

Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices

Article doi: 10.3791/61167
May 10th, 2020

Summary May 10th, 2020

Presented is a protocol for the isolation of human and animal ventricular cardiomyocytes from vibratome-cut myocardial slices. High yields of calcium-tolerant cells (up to 200 cells/mg) can be obtained from small amounts of tissue (<50 mg). The protocol is applicable to myocardium exposed to cold ischemia for up to 36 h.

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