Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / In Vivo Targeting of Neural Progenitor Cells in…
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

JoVE Journal Neuroscience
In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation
Ocular Kinematics Measured by In Vitro Stimulation of the Cranial Nerves in the Turtle
Journal (Neuroscience)
Ocular Kinematics Measured by In Vitro Stimulation of the Cranial Nerves in the Turtle
Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
Journal (Neuroscience)
Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
 

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

Article DOI: 10.3791/61171-v • 07:03 min • May 6th, 2020
May 6th, 2020 •
1,2, 1,3, 1, 4, 1
1Max Planck Institute of Molecular Cell Biology and Genetics, 2Human Technopole, 3Landesdirektion Sachsen, 4Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University

Chapters

Summary
Automatic Translation

Presented here is a protocol to perform genetic manipulation in the embryonic ferret brain using in utero electroporation. This method allows for targeting of neural progenitor cells in the neocortex in vivo.

Tags

In Vivo Targeting Neural Progenitor Cells Ferret Neocortex In Utero Electroporation Genetic Manipulation Animal Model Expansion And Folding Of Neocortex Spatial Specificity Temporal Specificity Neural Stem Cells Brain Development Glass Capillaries Micro Pipette Puller Diameter Adjustment Embryonic Day 33 DNA Concentration Fast Green Anesthesia Sedation Level Isoflurane Adverse Effects Liquid Form Handling Protective Equipment Surgery Canisters Subcutaneous Injection Analgesic Antibiotic Glucose Administration Eye Ointment Application Abdomen Shaving Skin Cleaning And Disinfection
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter