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Journal / Genetics / Methylierte RNA-Immunpräzipitierung Assay zur…
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Genetics

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JoVE Journal Genetics
Methylierte RNA-Immunpräzipitierung Assay zur Studie m5C Modifikation bei Arabidopsis
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Methylierte RNA-Immunpräzipitierung Assay zur Studie m5C Modifikation bei Arabidopsis

Article DOI: 10.3791/61231-v • 08:50 min • May 14th, 2020
May 14th, 2020 •
*1, *2, 3, 1
1Max Planck Institute of Molecular Plant Physiology, 2Department of Molecular Medicine, Medical School, University of Padua, 3Biologie de l'Ecole Normale Supérieure (IBENS), Paris, France
* These authors contributed equally

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Der methylierte RNA-Immunpräzipitationstest ist eine Antikörper-basierte Methode zur Anreicherung für methylierte RNA-Fragmente. Gekoppelt mit einer tiefen Sequenzierung führt dies zur Identifizierung von Transkripten, die die m5C-Modifikation tragen.

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Genetik Ausgabe 159 RNA-Methylierung 5-Methylcytosin (m5C) Immunpräzipitation (IP) Arabidopsis Epitranskriptomik mRNA RNA-Sequenzierung
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