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Developmental Biology

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JoVE Journal Developmental Biology
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Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging

Article DOI: 10.3791/61242-v • 09:00 min • June 20th, 2020
June 20th, 2020 •
1, 1, 1, 2, 1, 1, 3, 1
1Translational Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, 2Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 3Division of Pediatric Surgery, Department of Surgery, University of North Carolina at Chapel Hill

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Summary
Automatic Translation

The aim of this technique is ex vivo visualization of pulmonary arterial networks of early postnatal and adult mice through lung inflation and injection of a radio-opaque polymer-based compound via the pulmonary artery. Potential applications for casted tissues are also discussed.

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Vascular Casting Adult Mouse Lungs Early Postnatal Mouse Lungs Micro-CT Imaging Pulmonary Arterial Vasculature Soft Tissue Integrity Pulmonary-vascular Morphology Architecture Targeting Other Vascular Beds Xiphoid Process Rib Cage Diaphragm Lung And Trachea Exposure PE10 Tubing Sodium Nitroprusside PBS Heart Apex Penetration Curved Forceps 7-0 Silk
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