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Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue
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Biochemistry
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JoVE Journal Biochemistry
Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

DOI:
10.3791/61255-v

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10:12 min

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September 19, 2020

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, , ,
1Department of Biochemistry, School of Medicine,Shiraz University of Medical Sciences, 2Endocrinology and Metabolism Research Center, Nemazee Hospital,Shiraz University of Medical Sciences, 3English Language Department, Faculty of Paramedical Sciences,Shiraz University of Medical Sciences, 4Department of e-Learning in Medical Sciences, Virtual School and Center of Excellence in e-Learning,Shiraz University of Medical Sciences, 5Autophagy Research Center, School of Medicine,Shiraz University of Medical Sciences

Chapters

  • 00:00Introduction
  • 00:58The Removal of the Rat Pancreatic Tissue
  • 03:33RNA Extraction
  • 08:00Evaluating RNA Integrity with Denaturation Electrophoresis
  • 08:40Representative Results: The Evaluation of the RNA Integrity
  • 09:50Conclusion

Summary

Automatic Translation

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The purity and integrity of the isolated RNA is a vital step in RNA dependent assays. Here, we present a practical, rapid, and inexpensive method to extract RNA from a small quantity of undamaged pancreatic tissue.

Tags

Keywords: RNA ExtractionRapid MethodCost-effectiveAutolysisRNA StabilizationRNase InactivationRNA Integrity

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