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使用转基因钙处理器 Aequorin 前向基因屏幕,以识别钙信号中的新目标
JoVE Journal
Biology
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JoVE Journal Biology
Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling
DOI:

08:46 min

August 01, 2020

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Chapters

  • 00:00Introduction
  • 00:52EMS Mutagenesis (1-3 months)
  • 03:10High Throughput Screening (8 months)
  • 05:21Data Analysis and Results (1-3 months)
  • 06:46Conclusion and Final Thoughts

Summary

Automatic Translation

基于Ca2+高程的向前遗传屏幕作为读出,可以识别植物中钙依赖信号通路所涉及的遗传成分。

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