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Combined In Vivo Anatomical and Functional Tracing of Ventral Tegmental Area Glutamate Terminals in the Hippocampus
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Combined In Vivo Anatomical and Functional Tracing of Ventral Tegmental Area Glutamate Terminals in the Hippocampus

Article DOI: 10.3791/61282-v • 09:36 min • September 9th, 2020
September 9th, 2020 •
1, 1, 1
1Department of Comparative Biomedical Sciences, Louisiana State University School of Veterinary Medicine

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The current protocol demonstrates a simple method for tracing of ventral tegmental area (VTA) glutamate projections to the hippocampus. Photostimulation of VTA glutamate neurons was combined with CA1 recording to demonstrate how VTA glutamate terminals modulate CA1 putative pyramidal firing rate in vivo.

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In Vivo Anatomical Tracing Functional Tracing Ventral Tegmental Area Glutamate Terminals Hippocampus Neuroanatomical Tracing Electrophysiological Tracing Neurocircuits Adjunct Terminals Optogenetic Stimulation Extracellular Recording Sensory Function Motor Function Cognitive Function Stereotaxic Surgery Neural Electrodes Recording System Connection Visual Demonstration LED System In Vivo Optogenetics Amplifier LED Driver Anesthetized Mouse Heating Pad Surgical Area Iodine Solution Alcohol Lidocaine
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