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Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae
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Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae

Article DOI: 10.3791/61323-v • 07:24 min • June 11th, 2020
June 11th, 2020 •
1,2, *2, *1
1School of Biological Sciences, Monash University, 2Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA)
* These authors contributed equally

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Summary
Automatic Translation

Described here is a protocol that enables the colorimetric quantification of the amount of food eaten within a defined interval of time by Drosophila melanogaster larvae exposed to diets of different macronutrient quality. These assays are conducted in the context of a neuronal thermogenetic screen.

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Quantification Macronutrients Intake Thermogenetic Neuronal Screen Drosophila Larvae Neuronal Populations Nutritional Choices Proteins Carbohydrates Protocol Food Intake Genotypes Nutritional Conditions Embryo Collection Cages TripA1 Female Virgins Janelia GAL4 Males Mating Period Egg-laying Larval Density Plastic Lids Incubate
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