Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using <em>Drosophila</em> Larvae

Gon&#231;alo  M. Po&#231;as; Pedro  M. Domingos; Christen  K. Mirth

doi:10.3791/61323

Journal

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Neuroscience

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Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae

Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using <em>Drosophila</em> Larvae

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae

Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae

3,693 Views

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07:24 min

•

June 11, 2020

DOI:

10.3791/61323-v

DOI:

10.3791/61323-v

•

07:24 min

June 11, 2020

•

3655 Views

Gonçalo M. Poças^1,2, Pedro M. Domingos*², Christen K. Mirth*¹

¹School of Biological Sciences,Monash University ²Instituto de Tecnologia Química e Biológica António Xavier,Universidade Nova de Lisboa (ITQB NOVA)

Summary

Described here is a protocol that enables the colorimetric quantification of the amount of food eaten within a defined interval of time by Drosophila melanogaster larvae exposed to diets of different macronutrient quality. These assays are conducted in the context of a neuronal thermogenetic screen.