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Preparation of the Rat Vocal Fold for Neuromuscular Analyses
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Neuroscience
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JoVE Journal Neuroscience
Preparation of the Rat Vocal Fold for Neuromuscular Analyses

Preparation of the Rat Vocal Fold for Neuromuscular Analyses

DOI:
10.3791/61327-v

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07:17 min

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May 15, 2020

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, ,
1NYU Voice Center, Department of Otolaryngology-Head & Neck Surgery,New York University School of Medicine, 2Communicative Sciences and Disorders,New York University

Chapters

  • 00:05Introduction
  • 00:54Rat Larynx Dissection
  • 02:25Laryngeal Tissue Fixation and Flash-Freezing
  • 03:33Hemilarynx Cross-Sectional Plane Cryosectioning
  • 04:50Hemilarynx Longitudinal Plane Cryosectioning
  • 05:40Results: Representative Rat Vocal Fold Neuromuscular Analyses
  • 06:43Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes methods used to prepare rat vocal folds for histochemical neuromuscular study.

Tags

Rat Vocal FoldLaryngeal AnalysisNeuromuscular AnalysisCryosectioningIntrinsic Laryngeal MusclesThyroarytenoid MuscleLarynx DissectionFormaldehyde FixationSucrose Cryoprotection

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