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A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures

A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures

DOI:
10.3791/61330-v

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10:05 min

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March 18, 2021

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, , ,
1Instituto de Farmacologia e Neurociências,Faculdade de Medicina da Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes,Faculdade de Medicina da Universidade de Lisboa

Chapters

  • 00:04Introduction
  • 00:49Rhinal Cortex-Hippocampus Slice Preparation
  • 03:41Electrophysiological Recordings
  • 05:07Propidium Iodide (PI) Uptake Assay and Immunohistochemistry
  • 07:26Results: Epileptic-like Events, Neuronal Death and Gliosis are Observed in Rhinal Cortex-Hippocampus Organotypic Slices
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe the preparation of rhinal cortex-hippocampus organotypic slices. Under a gradual and controlled deprivation of serum, these slices depict evolving epileptic-like events and can be considered an ex vivo model of epileptogenesis. This system represents an excellent tool for monitoring the dynamics of spontaneous activity, as well as for assessing the progression of neuroinflammatory features throughout the course of epileptogenesis.

Tags

Keywords: EpilepsyEpileptogenesisRhinal CortexHippocampusOrganotypic Slice CulturesEx Vivo ModelSprague-DawleyGBSSOlfactory BulbsHippocampusDentate GyrusCA AreasEntorhinal CortexPerirhinal CortexCulture MediumCell Culture Incubator

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