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JoVE Journal Neuroscience
Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice
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Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice

Article DOI: 10.3791/61377-v • 08:58 min • July 2nd, 2020
July 2nd, 2020 •
1
1Department of Biology, Neurobiology, and Bio-X, Stanford University

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This is a protocol for acute slice preparation from adult and aging mouse hippocampi that takes advantage of transcardial perfusion and slice cutting with ice-cold NMDG-aCSF to reduce hypoxic damage to the tissue. The resulting slices stay healthy over many hours, and are suitable for long-term patch-clamp and field-recordings.

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Minimizing Hypoxia Hippocampal Slices Adult Mice Aging Mice Hypothermia Sodium-free Solutions Long-term Field-recordings Patch-clamp Studies Neurodegenerative Diseases Transcardial Perfusion Ice-cold Solution ACSF Solution NMDG-aCSF Vibrating Microtome Cutting Tray Recovery Chamber
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