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Journal / Developmental Biology / In vitro Neuromuskuläre Junction Induziert von…
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Developmental Biology

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JoVE Journal Developmental Biology
In vitro Neuromuskuläre Junction Induziert von humaninduzierten Pluripotenten Stammzellen
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In vitro Neuromuskuläre Junction Induziert von humaninduzierten Pluripotenten Stammzellen

Article DOI: 10.3791/61396-v • 06:01 min • December 3rd, 2020
December 3rd, 2020 •
1, 1,2, 3, 1
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, 2Department of Pediatrics, Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine, Taipei Medical University

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Hier stellen wir ein Protokoll zur Erzeugung von In-vitro-NMJs aus humaninduzierten pluripotenten Stammzellen (iPSCs) zur Verfügung. Diese Methode kann NMJs mit ausgereifter Morphologie und Funktion in 1 Monat in einem einzigen Brunnen induzieren. Die daraus resultierenden NMJs könnten potenziell verwendet werden, um verwandte Krankheiten zu modellieren, pathologische Mechanismen zu untersuchen oder medikamentöse Verbindungen für die Therapie zu untersuchen.

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Entwicklungsbiologie Ausgabe 166 iPSCs in vitro neuromuskuläre Kreuzung motorische Neuron Schwann-Zelle Myotube Kontraktion Curare
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