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Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids
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Chapters
- 00:04Introduction
- 00:40Testis Tissue Dissociation
- 03:35Preparation of Organoid Culture Dishes and Seeding of Cells
- 06:29Results: Self Assembled Organoids in 2D and 3D, ECM and ECM-free Culture Conditions
- 08:38Conclusion
Here, four methods for generating testicular organoids from primary neonatal murine testicular cells are described i.e., extracellular matrix (ECM) and ECM-free 2D and 3D culture environments. These techniques have multiple research applications and are especially useful for studying testicular development and physiology in vitro.
