Sertoli Cells
Sertoli Cells - Related Videos
11:11
Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
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Cited by 42 •
2016
Primary Sertoli cells are required for studying testis-immune privilege, signal transduction during inflammation or infection, and utilization of their immunoprotective properties. Here we describe an enzyme-based protocol for the isolation of highly purified primary Sertoli cells and peritubular cells from rat testes.
04:25
Isolation of Immune Regulatory Sertoli Cells from Mouse Seminiferous Tubules
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2025
This video demonstrates the isolation procedure of Sertoli cells from mouse seminiferous tubules, a component of the testes. Enzymatic digestion and repeated washes promote the release of Sertoli cells into the solution, which can be cultured further for proliferation and growth.
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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2025
This protocol describes the in vivo reprogramming of mouse cancer cells into type 1 dendritic-like cells within the tumor microenvironment through enforced expression of the transcription factors PU.1, IRF8, and BATF3.
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 8 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
02:47
Human ES cells: Starting Culture from Frozen Cells
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2006
Here we demonstrate how our lab begins a HuES human embryonic stem cell line culture from a frozen stock.
FAQs
Tight junctions between Sertoli cells form the blood-testis barrier, dividing the seminiferous tubules into distinct microenvironments. This organization helps regulate the conditions surrounding developing germ cells and limits their exposure to immune attack. As a result, the barrier supports orderly progression through spermatogenesis while protecting sperm-forming cells during development.
Sertoli cells respond to follicle-stimulating hormone and testosterone, linking endocrine signals to local control of sperm production. Their hormonal responsiveness helps coordinate support for developing germ cells and the broader regulatory environment of the seminiferous tubules. Sertoli-cell secretion of inhibin B also connects their activity with reproductive endocrine regulation.
Sertoli cells help organize the movement and progression of germ cells through spermatogenesis while removing cellular debris by phagocytosis. These activities maintain a functional environment within the seminiferous tubules. Coordinating cell development with debris removal supports the continuity and order of sperm production rather than allowing cellular remnants to accumulate.
Sertoli-cell research can show how changes in germ-cell support, barrier formation, hormonal responsiveness, or debris removal influence sperm production. Because these cells regulate several stages of the spermatogenic environment, their structure and function provide useful indicators of processes associated with fertility and of disorders that impair spermatogenesis.
A focused investigation can examine Sertoli-cell organization in seminiferous tubules, the tight junctions forming the blood-testis barrier, responses to follicle-stimulating hormone and testosterone, inhibin B secretion, and phagocytosis. Considering these features together reveals how structural, endocrine, protective, and cleanup functions contribute to the development of sperm.
Sertoli cells connect local events in the seminiferous tubules with broader reproductive regulation. Their support of developing germ cells, response to hormonal signals, and secretion of inhibin B make them relevant to testicular development and endocrine studies. Examining these roles can clarify how reproductive tissues coordinate sperm production with hormonal control.
Disruption of Sertoli-cell functions could compromise the microenvironments that developing germ cells require. Problems with tight-junction barriers, hormonal responses, inhibin B secretion, organization of germ-cell progression, or phagocytosis may interfere with normal sperm production. For this reason, Sertoli cells are important when investigating disorders associated with impaired spermatogenesis.