Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Modified Methods for Loading of High-Throughput…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Modified Methods for Loading of High-Throughput DNA Extraction Plates Reduce Potential for Contamination
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
Journal (Biology)
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
Journal (Biology)
Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
 

Modified Methods for Loading of High-Throughput DNA Extraction Plates Reduce Potential for Contamination

Article DOI: 10.3791/61405-v • 06:18 min • June 3rd, 2020
June 3rd, 2020 •
*1,2, *3,4
1Department of Ecosystem Science and Management, University of Wyoming, 2Program in Ecology, University of Wyoming, 3EPSCoR-IDEA, University of Wyoming, 4Department of Botany, University of Wyoming
* These authors contributed equally

Chapters

Summary
Automatic Translation

Current methods for loading 96-well plates for DNA extractions can be prone to contamination. We detail a new method for loading 96-well plates that reduces risk of cross-contamination among wells. Our method will help other researchers capitalize on the efficiency of high-throughput DNA extraction techniques and minimize risk of contamination.

Tags

High-throughput DNA Extraction Plates Contamination Reduction Loading Efficiency Sample Cross-contamination Methodology Microbiome Research Benchtop Sterilization Tools Sterilization Sub-sampling Sample ID Assignment Centrifuge Tubes Extraction Blank PCR Tubes 96-well Plate Preparation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter