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JoVE Journal Neuroscience
Uso De Las Neuronas Del Hipocampo Cultivadas Primarias Para Estudiar El Ensamblaje De Los Segmentos Iniciales Axón
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Uso De Las Neuronas Del Hipocampo Cultivadas Primarias Para Estudiar El Ensamblaje De Los Segmentos Iniciales Axón

Article DOI: 10.3791/61411-v • 06:53 min • February 12th, 2021
February 12th, 2021 •
1,2,3, 2,3
1School of Basic Medical Sciences, Xi'an Jiaotong University, 2Department of Cell Biology, Duke University, 3Department of Biochemistry, Duke University

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Aquí, se describe un protocolo para estudiar cuantitativamente el montaje y la estructura de los segmentos iniciales de axones (AIS) de las neuronas del hipocampo que carecen de AIS pre-montado debido a la ausencia de una anquiria gigante-G.

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Retracción Número 168 Anquirina gigante-G Segmento inicial de Axón Neuronas cultivadas primarias Transfección Imágenes Cuantificación de intensidad AIS
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