Journal
/
Developmental Biology
/
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

DOI:
10.3791/61446-v

•

08:47 min

•

May 15, 2020

•

, , ,
1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine,Penn State Hershey Cancer Institute

Chapters

  • 00:04Introduction
  • 00:58EB, NPC, and Neuron Differentiation
  • 03:00Fixation and Embedding
  • 04:24Immunofluorescence
  • 05:45Results: E14 Differentiation into EBs, NPCs, and Neurons
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the procedure for the in vitro differentiation of mouse embryonic stem cells into neuronal cells using the hanging drop method. Furthermore, we perform a comprehensive phenotypic analysis through RT-qPCR, immunofluorescence, RNA-seq, and flow cytometry.

Tags

Keywords: Mouse Embryonic Stem CellsNeuron DifferentiationNeural ProgenitorsEctodermal DevelopmentHanging Drop CultureEmbryoid BodyNeural Progenitor CellsRetinoic AcidImmunocytochemistry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61446?article_type=v

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture
check_url/61446?article_type=v

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
check_url/61446?article_type=v

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
check_url/61446?article_type=v

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
check_url/61446?article_type=v

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
check_url/61446?article_type=v

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
check_url/61446?article_type=v

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
check_url/61446?article_type=v

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
check_url/61446?article_type=v

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
check_url/61446?article_type=v

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Read Article