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Visualization of DNA Repair Proteins Interaction by Immunofluorescence
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Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Article DOI: 10.3791/61447-v • 07:55 min • June 26th, 2020
June 26th, 2020 •
1,2, 1,2
1Department of Biochemistry and Structural Biology, University of Texas Health Science Center at San Antonio, 2Mays Cancer Center at UT Health San Antonio MD Anderson Cancer Center

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Summary
Automatic Translation

Following DNA damage, human cells activate essential repair pathways to restore the integrity of their genome. Here, we describe the method of indirect immunofluorescence as a means to detect DNA repair proteins, analyze their spatial and temporal recruitment, and help interrogate protein-protein interaction at the sites of DNA damage.

Tags

Visualization DNA Repair Proteins Immunofluorescence Protein-protein Interactions DNA Damage Foci Colocalization Complex Formation HeLa Cells Gamma Irradiation NDB Incubation Time PBS
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